I found myself wanting to quote half a dozen posts because yes, yes, yes- if you are in an endemic area like New England, your horses will certainly test for some Lyme antibodies. Like others have said, the Cornell multiplex is the way to go for maximum info to help you decide what to do- OspC (active/early infection) might get treated in a horse who is acting symptomatic, OspF (chronic) probably won’t get treated. I’ve had 4 Morgans treated over the years: protocol for an acute/active infection was 5 days IV tetracycline with at least 30 days oral doxy. For one mare in her mid-20s, she lost so much muscle tone/topline through infection and treatment that she basically retired, though was no longer so uncomfortable in terms of symptoms. With the others, they continued on in work with no notable impact.
Using the dog vaccine doesn’t feel pointless to me, as I haven’t had an active infection since vaccination. That said, you are right that you need to do it more than once. Our vet’s protocol is 3 doses on initial vaccination, then recommended every 6 months after. Spring round before ticks emerge, which is getting harder as winters warm, even in Vermont, because ticks aren’t reliably killed off over the winter any more. Note that vaccinated horses will show elevated OspA on Cornell’s multiplex.
The vaccine is pretty much my only preventative measure- I don’t see any topical sprays or spot treatments work in a meaningful way. I wish there was an oral treatment for people and horses like there is for dogs. The dog preventative IS effective in killing them upon being bitten.
And unfortunately, as someone else pointed out, the dog Lyme vaccine won’t protect against other nasty tick borne diseases. One of my mares was a little off her feed this fall and scared me to death presenting with a 106*F fever. That’s not a typo, 106*F was her actual temp read on multiple thermometers. I truly didn’t know they could survive that. She was treated aggressively for anaplasmosis. I’ve never seen a horse feel so bad, but she recovered beautifully- other than developing a real aversion to syringing (had to crush and syringe in antibiotics for a couple of weeks because she didn’t want to eat anything but hay while she was so sick). Hard to blame her, it was a tough couple of weeks.