The 24 behaviors:
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Repeated changes of head position (up/down), not in rhythm with the trot
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Head tilted or tilting repeatedly
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Head in front of vertical (>30°) for ≥10 s
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Head behind vertical (>10°) for ≥10 s
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Head position changes regularly, tossed or twisted from side to side, corrected constantly
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Ears rotated back behind vertical or flat (both or one only) ≥5 s; repeatedly lay flat
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Eye lids closed or half closed for 2–5 s; frequent blinking
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Sclera exposed repeatedly
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Intense stare (glazed expression, ‘zoned out’) for ≥5 s
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Mouth opening ± shutting repeatedly with separation of teeth, for ≥10 s
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Tongue exposed, protruding or hanging out, and/or moving in and out repeatedly
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Bit pulled through the mouth on one side (left or right), repeatedly
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Tail clamped tightly to middle or held to one side
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Tail swishing large movements: repeatedly up and down/side to side/circular; repeatedly during transitions
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A rushed gait (frequency of trot steps > 40/15 s); irregular rhythm in trot or canter; repeated changes of speed in trot or canter
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Gait too slow (frequency of trot steps < 35/15 s); passage-like trot
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Hindlimbs do not follow tracks of forelimbs but repeatedly deviated to left or right; on three tracks in trot or canter
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Canter repeated leg changes in front and/or behind; repeated strike off on wrong leg; disunited
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Spontaneous changes of gait (e.g., breaks from canter to trot, or trot to canter)
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Stumbles or trips more than once; repeated bilateral hindlimb toe drag
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Sudden change of direction, against rider’s direction; spooking
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Reluctance to move forwards (has to be kicked ± verbal encouragement), stops spontaneously
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Rearing (both forelimbs off the ground)
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Bucking or kicking backwards (one or both hindlimbs)
“A total behaviour score of ≥8 (out of 24) is likely to indicate the presence of musculoskeletal pain.”