determining what makes up a frozen dose

I understand 200 million progressive motility is the World Breeding Federation standard. Has anyone had to reconsider what makes a dose of frozen likely to result in a pregnancy? Stallion is deceased, 8 x .5 mil straw doses, frozen has always worked well. The statistics I am vague on now as to how many million progressive sperm per dose -His may have been between 300-400 million progressive motility per dose.I also have been told frozen is a strange product - as there is some Unknown (U) factor involved apart from numbers where some stallions frozen works well and others even w high progressive motility does not work. His works. If I find a decent fertile mare and find a skilled vet and I may want to experiment.Experience appreciated.
TIA

You are correct that there are stallions with super post thaw motility and don’t work well along with vice versa. Extenders, seminal fluid left over, debris or what have you can affect breeding success. Straws/dose really has to be determined by concentration and post thaw and morphology. Ideally the best is to find out from who froze it or have it tested. We’ve had a lot of success with frozen as have our clients. It is important to have an experienced vet, fertile mare and breeding within 6 hours of ovulation. I see so many different studies and success rates on frozen its hard to see a number but for sure its less successful, harder and more management on the mare than fresh.

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Thanks for confirming . It was frozen by a top reproduction place…and the numbers per straw are available, I just need to retrieve them. I am trying to determine whether one could do 4 or 6 (.5 ) straws (if it is 300-400 million per dose) which might put it below 200 million progressive motility sperm for the insemination dose…still reasonably expect a pregnancy if all goes well?

I am curious when I hear folks successfully split doses or use 1 or 2 straws (may be bigger straws??) exactly how many millions of progressive motility sperm they are working with! :slight_smile: TIA

(P…S. This is family owned stallion frozen so I am not trying to maximize a contractually purchased dose-which I understand may violate some very restrictively written contracts-just trying to stretch out what we got.)

My friend had luck with her mare from only 2 straws! The guy was very on it he had the best rating in all of so cal. My friend found him after the first “specialist” used 6 with no luck finding out the mare wasn’t even ovulating! She was pissed so made sure to get the best after that and it sure worked for her! She used Frozen.