Do you know if it’s possible to determine whether two dogs are full siblings using the DNA of the two dogs in question as well as the mothers DNA (if required)? We don’t have the father’s DNA. These are purebred puppies produced by a repeat mating, that have unexpectedly turned out very differently indeed from what was expected. If this is possible, can anyone recommend a company that will do these DNA tests?
Many thanks 
I think if you ran an Embark on both of them, it would show their degree of relatedness.
Most of the parentage testing companies request DNA samples from sire, dam, and the pup(pies) - here’s one that looks like they might be able to work around the request for sire’s DNA. Looks like it requires contacting them for more info. Maybe worth investigating? https://vetdnacenter.com/dna-tests/canine-dna-testing/canine-parentage-testing/
Dont waste your time or money on Embark. They are not designed for parentage. Degree of relatedness is very ambiguous.
AKC does a lot of DNA testing for multiple sired litters. Id call them and ask.
Thanks for the replies! Embark would not have the type of result I require but the other company would if they would be happy to use a full siblings DNA rather than the sire. Thank you so much for the link. These are not AKC and our KC do not have DNA on file, plus the sire is from another different KC that also does not DNA test. How much easier it is with horses!
My point was that AKC probably fields this question often so they can tell you what is necessary for proof of paternity.
The other company may also be able to answer the question.
This is one good reason to have DNA on file for all breeding dogs. I believe AKC also requires the dog to have a permanent identification in order to have DNA on file - microchip or tattoo - which makes it even harder to substitute the wrong dog for breeding. (DNA is not required by AKC but many parent clubs require it for various events.)
Can you not get the DNA of the supposed sire?
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