[QUOTE=Kyzteke;7043663]
This is a question that has been debated for as long as I’ve been breeding: where is the best place for that semen: in the cooler or in the mare?
I choose “in the cooler” because, according to the research I am aware of, the mare is actually a “hostile” place for the semen. IMHO the die off rate would be lower if the sperm is kept in a non-stress environment, cooled down to lessen metabolism and given nourishment (the extender).
I admittedly am not aware of any studies that actually PROVE this, but it’s the conclusion I’ve come to.
It could be that when you check the semen stored in the cooler 24 hrs later, you have only (let’s say) 50% FM when you had 70% on collection. But (for all we know) there is only 30% surviving mobile sperm from Dose #1 24 hrs. after that dose was placed in the mare.
So if you DO need a 2nd dose (because mares are mares), it’s just my thinking that the dose stored in the cooler is in better shape.
But I may well be wrong…
Of course, I have also factored in the fact that the semen is irritating to the uterus as well, so the volume is best kept under control.
But again, this is something that breeders & vets argue about constantly…[/QUOTE]
20 years ago the general consensus was that semen was best stored and second dose used the following day if mare hadn’t ovulated. However more recent studies seems to indicate that the best place for semen is in the mare. The semen switches off and conserves energy until the mare ovulates.
We only use 15mls and if the mare hasn’t ovulated within 48 hours we will reinseminate with a fresh collection. If the semen is still alive in the fridge then it’s still alive in the mare so there seems little benefit in using semen from the same collection that has already been used.
All the Dutch and German stallion stations only ever send out 15mls so we can assume that they know what works best considering the vast numbers of mares bred each year and the consensus is definitely less is more.